The bd accuri c6 has the sensitivity, reliability, and capabilities needed to bring flow cytometry to the next. However, the device driver is 32bit compatible only. Small benchtop flow cytometers from stratedigm, inc. Bd accuri c6 flow cytometer instrument manual this section provides details on installing bd accuri c6 software and the bd accuri c6 flow cytometer driver. Bd accuri c6 software sample grid cytometer status fluidics controls run criteria threshold and. The bd accuri c6 flow cytometer along with the bd cytometric bead array reagent sets, provides researchers a powerful flow cytometry toolset for cell analysis. From basic to more challenging applications, the bd accuri c6 plus personal flow cytometer is a powerful benchtop tool that provides you with the versatility and the analytical power of a cytometer twice its size. Bd accuri c6 plus flow cytometer with its compact 11 x 14. A practical guide for using flow cytometry and cell sorting, including extensive discussion on hardware, suppliers, reagents, and software. The bd accuri c6 flow cytometer is easy to use and allows student access to the science of flow cytometry. Simplifying complex flow cytometric analyses university of helsinki. Optimize your flow cytometry best practices for sample preparation, staining and analysis duration. The bd accuri c6 is a digital basic flow cytometer, which is equipped with a blue 488nm and a red 640nm lasers, two light scatter detectors fsc and ssc, and four fluorescence detectors. The bd accuri c6 plus flow cytometer is easy to use, simple to maintain and affordable.
Blue lion biotech flow cytometers bd accuri c6 flow. Bd accuri c6 software sample grid cytometer status fluidics controls run criteria threshold and compensation real time updates analysis and gating tools regions quadrant markers histograms. Page 1 bd csampler installation guide install and validate the bd accuri c6 flow cytometer with 6 and 8peak beads prior to installing the bd csampler. Bd accuri c6 with plate handler acquisition april 2016 multiwell auto collect sample important only run this protocol after you have performed cytometer setupverification protocol on your bd accuri. For what it is worth, i have had bad experiences in general with bd as a company and bd accuri flow cytometers. In bd accuri c6 software, the range of axes viewed is set using the zoom. Cited in over 9,000 publications, the bd accuri c6 plus enables you to go beyond your current research potential.
Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new. Take a leap in flow cytometry with the bd accuri c6 plus. Enhanced sensitivity and reliability bring flow cytometry within reach for new and. Since the initial commercialization of flow cytometry fc and fluorescence activated cell sorting facs in 1968, they have undergone significant improvements. The bd accuri control software for the original bd accuri machines will install and run on both 32 and 64 bit windows versions. The c6 uses two lasers, red and blue, to record events and has 4 fluorescence channels for. For the d accuri 6, use the pe channel fl2 for the reporter and the apc. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. Bd csampler accessory kit shipping contents bd csampler module bd csampler mat bd csampler mounting bolts 3 module. Bd accuri c6 plus software features an intuitive user interface. Calibrationshould be performedin the same tube type as the experimental sample. All reagents and kits are compatible with both bd accuri c6 plus and accuri c6 flow cytometer systems.
Breakthrough with the bd accuri c6 plus cell analyzer take a leap in flow cytometry with the bd accuri c6 plus. Bd accuri c6 system 32bit software is not forward compatible with bd accuri c6 plus 64bit software and the fcs 3. It may be suitable for those labs that require more routine applications, andor dont require a large number of fluorescence. Bd accuri c6 software controls the bd accuri c6 flow cytometer system in order to acquire data, generate statistics, and analyze results.
This portable and compact system holds the analytical power of a cytometer twice its size. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd. Lightweight, with a small footprint and straightforward maintenance, its often the cytometer of choice. Even new flow cytometry users find it so easy to learn and use that. Accuri c6 plus bd biosciences this desktop flow cytometer is the newest generation of the bd accuri platform. Current extended warranties will not be affected and will be supported until they expire. All samples were collected and analyzed on a bd accuri c6 flow cytometer using bd accuri c6 software. The default configuration of the bd accuri c6 is similar to the facscalibur2 3blue 1red with filters optimized for the detection of fluorochromes such as fitc, pe, percp, and apc. The bd accuri c6 flow cytometer reports arithmetic means. A guide to absolute counting using the bd accuri c6 flow cytometer bd biosciences august 2011 page 3 5. To install bd accuri c6 software, run bdaccuric6softwareinstaller. Using the bd accuri c6 cytometer for rapid and accurate.
Remove and discard the black sip cover and place a tube containing at least 2ml of filtered, deionized water on the sip. Since the initial commercialization of flow cytometry fc and. This small but powerful accuri c6 comes computer loaded with bd accuri c6 plus software. The dna histograms produced by the bd accuri c6 display excellent peak cvs, and this instrument, uniquely, can provide simultaneous. The accuri c6 offers a budget friendly option for a bench top flow cytometer. Bd biosciences accuri c6 personal flow cytometer overview. It is small enough to easily fit on a benchtop, and can be placed in a laminar flow hood. Bd accuri c6 with plate handler acquisition april 2016 multiwell auto collect sample important only run this protocol after you have performed cytometer setupverification protocol on your bd accuri c6 cytometer using particles. When combined with the portfolio of bd reagents and bestin. The accuri system was engineered from the start to be compact, powerful and userfriendly, while also being affordable by most research laboratories.
For the d accuri 6, use the pe channel fl2 for the reporter and the apc channel fl4 for beads classification. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Bd accuri c6 plus software files can be exported in fcs 3. After the cytometer is shut down, leave the cytometer off for at least 30 minutes, for a more thor ough. Setup procedure for d accuri 6 this part of the guide applies to bd digital flow cytometers using d accuri 6 software. Breakthrough with the bd accuri c6 plus cell analyzer. May 08, 2019 accuri c6 plus flow cytometer duration. The bd accuri c6 personal flow cytometer brings immunology and stem cell studies to the benchtop. Fcs files from flow cytometers with 18bit or higher digital signal processing, such as the bd accuri c6 flow cytometer system, often require adjustment of flowjos scaling factors to properly visualize the data. New generation accuri makes flow cytometry even more within reach. After the cytometer is shut down, leave the cytometer off for at least 30 minutes, for a more thor ough cleaning.
Small and easily transportable, the bd accuri c6 plus cytometer measures 11 x 14. Bd accuri c6 flow cytometer system and bd csampler includes bd accuri c6 flow cytometer, c6 software, workstation computer, standard optical filters, reagent containers, complimentary c6 flow cytometer starter kit, bd csampler accessory, and oneyear warranty. Two additional features further streamline the process. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic. Bd accuri c6 flow cytometer the bd accuri c6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. With two lasers, two scatter detectors, and four fluorescence detectors, it can handle most common assays with ease. Accuri c6 is a reliable instrument for hsc enumeration in fresh samples, using both volumetric and beadbased approaches, although the volumetric protocol on cord blood samples needs to be improved. Jul 07, 2015 a new lab toy to make it big in the last 510 years is the accuri c6 cytometer now under the bd umbrella, a lowcost instrument in comparison to the big boys. Any one have good or bad experiences with the new bd. The accuri c6 is easy to use, does not require profound knowledge of flow cytometry and could be employed in an urgent setting.
Service for bd accuri c6 systems and their related options will continue until april 15, 2021. The bd accuri c6 has the sensitivity, reliability, and capabilities needed to bring flow cytometry to the next level. Nov 09, 2017 optimize your flow cytometry best practices for sample preparation, staining and analysis duration. Fcs files from flow cytometers with 18bit or higher digital signal processing, such as the bd accuri c6 flow cytometer system, often require adjustment of flowjos. Samples were collected on the bd accuri c6 flow cytometer using the kit template and analyzed using bd accuri c6 software. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results. Bd has discontinued selling the bd accuri c6 flow cytometer as of december 31, 2016. Ive recently started doing flow cytometry bd accuri c6 in a core facility at my university, but my lab doesnt have any softtware to analyze the data. The c6 uses two lasers, red and blue, to record events and has 4 fluorescence channels for a wide range of fluorescence detection. Used flow cytometers bd facsaria, facscanto and accuri c6.
Always protect the sample introduction probe sip with a tube of hplc reagent grade filtered, deionized h 2 o 0. Usa special offer bd accuri c6 plus flow cytometer system and bd csampler includes bd accuri c6 plus flow cytometer, c6 plus software, workstation computer, standard optical filters, reagent. The intuitive interface of the software guides the user through workflows. Bd biosciences bd accuri c6 plus community, manuals and. Many bd accuri c6 customers can begin collecting and analyzing data with the help of a quick start guide. Inspect the bd csampler accessory kit contents to ensure delivery of all components.
Instrument includes a c sampler, computer, software, validation report and warranty. Bd accuri c6 flow cytometry system available for sale. However, the accompanying software isnt the best, but it is free to use and does the job. Professors can request their students to be trained on the accuri student cytometer by sending an email to. Bd accuri c6 flow cytometer for the analysis of plant genome sizes. The bd accuri c6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable.
Accuri cytometers was founded to address a compelling need. Instrument includes a csampler, computer, software, validation report and warranty. The bd accuri c6 plus offers simplified workflows and readytouse templates for performing a wide range of applications in flow cytometry. Long story short, during my postdoc we had constant problems with our bd accuri c6. Purchase a stratedigm benchtop flow cytometer and be confident that you have made the best possible flow cytometry investment. Bd accuri c6 software has an intuitive user interface that was developed based on hundreds of hours observing researchers using flow cytometers. The accuri c6 flow cytometer is a personal cytometer from bd biosciences. Within 5 minutes of completing the water run, place a calibration sample on the sip. Bd biosciences accuri c6 plus flow cytometer duration. Turn on the computer and log in with your ucf info. This protocol also assumes a completed assay in a 96well plate, with particles suspended in run buffer. The analytical power and versatility of todays laserbased flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. The bd accuri control software for the original bd accuri machines will install and run on both 32 and 64.
Enhanced sensitivity, reliability, and capabilities bring flow. The bd accuri c6 flow cytometer an affordable, fullfeatured, easytouse flow cytometer. Bd accuri c6 plus flow cytometer technical specifiations. It is a personal flow cytometer that brings cell analysis within reach by easy to use, simple to maintain, and affordable. The bd accuri c6 software installer is on the cd that came with the c6. The accuri c6 flow cytometer does not produce aerosols during normal operation. Bd biosciences accuri c6 personal flow cytometer software. Usa special offer bd accuri c6 plus flow cytometer system and bd csampler includes bd accuri c6 plus flow cytometer, c6 plus software, workstation computer, standard optical filters, reagent containers, complimentary c6 plus flow cytometer starter kit, bd csampler accessory, and oneyear warranty.
Bd accuri c6 plus flow cytometer from bd biosciences. This portable and compact system holds the analytical power of a cytometer twice. The dna histograms produced by the bd accuri c6 display excellent peak cvs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear dna content spanning at least two orders of magnitude a range of 0. A personal research flow cytometer that is easy to use, simple to maintain, and affordable. Bd accuri c6 flow cytometer system and bd csampler includes bd accuri c6 flow cytometer, c6 software, workstation computer, standard optical filters, reagent containers, complimentary c6 flow. In most cases, bd accuri c6 software will be preinstalled. Bd accuri c6 flow cytometer from bd biosciences selectscience. Evaluation of an easy and affordable flow cytometer for.
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